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KMID : 1146920180480040477
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Journal of Pharmaceutical Investigation
2018 Volume.48 No. 4 p.477 ~ p.486
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Effect of Marsilea quadrifolia (L.) on carbohydrate metabolic enzymes in alloxan induced diabetic rats
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Karikalan Gopalakrishnan
Rajangam Udayakumar
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Abstract
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Marsilea quadrifolia is an indigenous medicinal plant and it has a folk reputation in central and southern India as hypoglycemic agent. The present study was aimed to evaluate the effect of methanolic leaf and stem extracts of Marsilea quadrifolia on carbohydrate metabolic enzymes in alloxan induced diabetic rats. In this study, the blood glucose level was increased in alloxan induced diabetic rats when compared to normal control rats. The decreased level of blood glucose was observed in diabetic rats treated with leaf and stem of M. quadrifolia. The activities of hexokinase, pyruvate kinase, lactate dehydrogenase, glucose-6-phosphatase, fructose 1,6-bisphosphatase and glucose-6-phosphate dehydrogenase in liver and kidney and glycogen content, glycogen synthase and glycogen phosphorylase in liver were analysed. Glibenclamide was used as a reference drug in the present investigation. The activities of hexokinase, pyruvate kinase and glucose-6-phosphate dehydrogenase were significantly decreased and the activities of lactate dehydrogenase, glucose-6-phosphatase and fructose 1,6-bisphosphatase were significantly increased in liver and kidney of alloxan induced diabetic rats when compared to normal control rats. Oral administration of methanolic leaf and stem extracts of M. quadrifolia (300 mg/kg bw) to diabetic rats for 45 days showed significantly reverted levels of above mentioned enzymes. The levels of glycogen and glycogen synthase in liver were significantly decreased and the glycogen phosphorylase activity was significantly increased in diabetic rats when compared to normal control rats. The diabetic rats treated with leaf and stem extracts of M. quadrifolia reverted the levels of glycogen, glycogen synthase and glycogen phosphorylase to near normal. The present study clearly indicates that the methanolic leaf and stem extracts of M. quadrifolia possesses potent antidiabetic activity.
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KEYWORD
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Antidiabetic, Alloxan monohydrate, Hypoglycemic, Marsilea quadrifolia
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